SfiI

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0123S     -20       SfiI R0123SVIAL -20 1 x 0.15 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
R0123L     -20       SfiI R0123LVIAL -20 1 x 0.75 ml 20,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Companion Products

• dam^-/dcm^- Competent E. coli

Materials Sold Separately

• rCutSmart™ Buffer

1. SfiI requires two copies of its recognition sequence for cleavage to occur. The two sites can be on either the same or different DNA molecules. Wertzell L.M. et al., (1995) J. Mol. Biol. 248:581-595.
   
   For more information and recommendations on working with enzymes requiring multi-sites, please visit Restriction Enzyme Cleavage: ‘single-site’ enzymes and ‘multi-site’ enzymes
2. For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
3. Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
4. Impaired by overlapping dcm methylation or blocked by some combinations and overlapping CpG methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Restriction enzymes requiring multi-sites for efficient cleavage
• Single Letter Codes
• Site Preferences
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Sequences
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Enzymes with Multiple Recognition Sequences
• Frequencies of Restriction Sites
• Interrupted Palindromes
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes
• Why Choose Recombinant Enzymes?

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. What is the minimum distance needed between two SfiI sites? Can they follow each other directly?
2. Can I achieve digestion of a plasmid with a single SfiI site?
3. Do degenerate recognition sites need to be palindromic?
4. How can this enzyme be inactivated?
5. Why isn't the enzyme cutting completely?
6. Can I add an oligo to my reaction so that two sites are available for efficient cutting?
7. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
8. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
9. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
10. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

• Restriction Enzyme Troubleshooting Guide

• Restriction Enzyme Cleavage: ‘single-site’ enzymes and ‘multi-site’ enzymes

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0123S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0123S_L_v1_0271403
• R0123S_L_v1_0261211
• R0123S_L_v1_0261303
• R0123S_L_v1_0261309
• R0123S_L_v1_0271408
• R0123S_L_v1_0271501
• R0123S_L_v1_0271507
• R0123S_L_v1_0271512
• R0123S_L_v1_0291601
• R0123S_L_v1_0291607
• R0123S_L_v1_0291701
• R0123S_L_v1_0291711
• R0123L_v1_10013031
• R0123S_v1_10013032
• R0123L_v1_10027011
• R0123S_v1_10027015
• R0123L_v1_10035353
• R0123S_v1_10035355
• R0123S_v1_10050034
• R0123L_v1_10058102
• R0123S_v1_10058731
• R0123L_v1_10065029
• R0123S_v1_10063457
• R0123L_v1_10072936
• R0123S_v1_10077059
• R0123S_v1_10082435
• R0123L_v1_10084471
• R0123S_v1_10084472
• R0123S_v1_10093175
• R0123S_v1_10096365
• R0123L_v1_10096366
• R0123L_v1_10123858
• R0123S_v1_10135686
• R0123S_v1_10140927
• R0123L_v1_10140925
• R0123S_v1_10158745
• R0123L_v1_10164922
• R0123L_v1_10175829
• R0123S_v1_10175827
• R0123S_v1_10182605
• R0123S_v1_10192457
• R0123L_v1_10195704
• R0123S_v1_10204877
• R0123S_v1_10210115
• R0123L_v1_10213968
• R0123L_v1_10241223
• R0123S_v1_10241222
• R0123L_v1_10260280
• R0123S_v1_10260994
• R0123L_v1_10303379
• R0123S_v1_10303380
• R0123S_v1_10312915
• R0123S_v1_10328779

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• SfiI
• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.