Home Competent Cells dam^–/dcm^– Competent E. coli

dam^–/dcm^– Competent E. coli

Methyltransferase deficient E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation

No dry ice surcharge on competent cell shipments
Outgrowth medium included
Free of animal products

Catalog #	Cat #	Concentration	Conc	Size	List Price
C2925H	Not Applicable	20 x 0.05 ml	$305.00	$305.00	Sign In Sign In
C2925I	Not Applicable	6 x 0.2 ml	$234.00	$234.00	Sign In Sign In

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Product Information

Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam^- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam⁺ strain unless there is a specific need for DNA free of Dam or Dcm methylation.

Highlights

Grow plasmids free of Dam and Dcm methylation
Free of animal products
Phage T1 resistant (fhuA31)
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
K12 Strain

Transformation Efficiency

1-3 x 10⁶ cfu/μg pUC19 DNA

Genotype

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) Tet^S endA1 rspL136 (Str^R) dam13::Tn9 (Cam^R) xylA-5 mtl-1 thi-1 mcrB1 hsdR2

This product is related to the following categories:: Cloning Competent Cell Strains

This product can be used in the following applications:: High-throughput cloning and automation solutions,; Transformation

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

C2925H

-80

pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml	Not Applicable
dam^–/dcm^– Competent E. coli	C2925HVIAL	-80	20 x 0.05 ml	Not Applicable

C2925I

-80

pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml	Not Applicable
dam^–/dcm^– Competent E. coli	C2925IVIAL	-80	6 x 0.2 ml	Not Applicable

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 µg/ml
Carbenicillin	100 µg/ml
Kanamycin	30 µg/ml
Tetracycline	15 µg/ml

Shipping Notes

Ships on dry ice

Antibiotic Resistance

Advantages and Features
Features
- Dam/Dcm methyltransferase free plasmid growth
Application Features

Effect of heat shock time on dam^-/dcm^- competent E. coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.

Effect of DNA incubation time on dam-/dcm- competent E. coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Effect of outgrowth medium on transformation efficiency:
50 μl of -/dcm^-competentE.coli was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB SOC outgrowth medium delivers the highest transformation efficiency.
Related Products
Companion Products
Materials Sold Separately
- SOC Outgrowth Medium
Product Notes
1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.