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NheI

This product has been discontinued and is replaced with the High-Fidelity version, NheI-HF (NEB #R3131)

Catalog # R0131 was discontinued on March 17, 2021

NEB restriction endonuclease that recognizes the sequence G^CTAG_C

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3131) supplied with CutSmart^® Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Cut Site: G/CTAGC

Product Information
NheI has a High Fidelity version NheI-HF® (NEB #R3131).

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999).

This product is related to the following categories:

Discontinued (<3 years)
- Properties & Usage
  Unit Definition
  One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
  Reaction Conditions
  
  1X NEBuffer™ 2.1
  Incubate at 37°C
  
  1X NEBuffer™ 2.1
  50 mM NaCl
  10 mM Tris-HCl
  10 mM MgCl₂
  100 µg/ml BSA
  (pH 7.9 @ 25°C)
  
  Activity in NEBuffers
  NEBuffer™ 1.1: 100%
  NEBuffer™ 2.1: 100%
  NEBuffer™ 3.1: 10%
  
  Diluent Compatibility
  
  Diluent C
  
  Storage Buffer
  
  10 mM Tris-HCl
  250 mM NaCl
  1 mM DTT
  0.1 mM EDTA
  200 µg/ml BSA
  50% Glycerol
  0.15% Triton® X-100
  pH 7.4 @ 25°C
  
  Heat Inactivation
  65°C for 20 minutes
  Methylation Sensitivity
  
  dam methylation: Not Sensitive
  dcm methylation: Not Sensitive
  CpG Methylation: Blocked by Some Combinations of Overlapping
- Related Products
  Companion Products
  
  NheI-HF®
  
  Nuclease-free Water
  
  Materials Sold Separately
  
  Gel Loading Dye, Purple (6X)
  
  NEBuffer 2.1
- Product Notes
  Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or XbaI.
  
  Inhibited by salt concentrations > 100 mM.
  
  This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
  
  Blocked by some combinations of overlapping CpG methylation.
Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Digest Protocol
  
  Double Digest Protocol with Standard Restriction Enzymes
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Effects of CpG Methylation on Restriction Enzyme Cleavage
  
  Heat Inactivation
  
  NEBuffer Activity/Performance Chart with Restriction Enzymes
  
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Site Preferences
  
  Star Activity
  
  Traditional Cloning Quick Guide
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Sequences
  
  Cleavage of Supercoiled DNA
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoelectric Points (pI) for Restriction Enzymes
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Recleavable Filled-in 5' Overhangs
  
  Time-Saver™ Qualified Enzymes
  
  Why Choose Recombinant Enzymes?
- Web Tools
  Competitor Cross-Reference Tool
  
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter™ v3.0
  
  NEBioCalculator®
  
  REBASE®
FAQs & Troubleshooting
- FAQs
  Is NheI affected by methylation?
  
  Are more units of NheI required to cut supercoiled DNA than lambda DNA?
  
  Is NheI inhibited by salt?
  
  Is NheI active at 25°C?
  
  Does NheI produce commonly used compatible ends?
  
  Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  
  Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
- Troubleshooting
  Restriction Enzyme Troubleshooting Guide
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0131M_v1
  
  R0131S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0131M_v1_0281306
  
  R0131M_v1_0291312
  
  R0131S_L_v1_0281301
  
  R0131S_L_v1_0291306
  
  R0131S_L_v1_0291312
  
  R0131M_v1_0271301
  
  R0131M_v1_0291405
  
  R0131S_L_v1_0271301
  
  R0131S_L_v1_0291405
  
  R0131M_v1_0291410
  
  R0131S_L_v1_0291410
  
  R0131S_L_v1_0291504
  
  R0131M_v1_0291504
  
  R0131S_L_v1_0291509
  
  R0131S_L_v1_0291604
  
  R0131M_v1_0291509
  
  R0131M_v1_0291605
  
  R0131S_L_v1_0291608
  
  R0131S_L_v1_0291611
  
  R0131S_L_v1_0291701
  
  R0131M_v1_0291705
  
  R0131S_L_v1_0291704
  
  R0131M_v1_0291711
  
  R0131S_L_v1_0291711
  
  R0131M_v1_0291701
  
  R0131M_v1_10011628
  
  R0131L_v1_10008860
  
  R0131S_v1_10015942
  
  R0131S_v1_10020681
  
  R0131S_v1_10027617
  
  R0131M_v1_10032186
  
  R0131S_v1_10035482
  
  R0131L_v1_10027620
  
  R0131L_v1_10042389
  
  R0131S_v1_10042391
  
  R0131M_v1_10053501
  
  R0131S_v1_10057181
  
  R0131L_v1_10063024
  
  R0131S_v1_10063025
  
  R0131M_v1_10071487
  
  R0131S_v1_10069390
  
  R0131S_v1_10078814
  
  R0131S_v1_10085530
  
  R0131S_v1_10091136
  
  R0131L_v1_10088050
  
  R0131M_v1_10088048
  
  R0131S_v1_10091579
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NheI
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Trademarks

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