AleI-v2

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0685S     -20       AleI-v2 R0685SVIAL -20 1 x 0.05 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
R0685L     -20       AleI-v2 R0685LVIAL -20 1 x 0.25 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Companion Products

• Nuclease-free Water
• Gel Loading Dye, Purple (6X)

Materials Sold Separately

• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

1. AleI-v2 is an isoschizomer of OliI.
2. This enzyme is impaired by Cpg methylation. More information can be found at "Dam-Dcm and CpG Methylation."
   
3. AleI-v2 has the same specificity as AleI (NEB #R0634), but it has been engineered for increased stability at -20C.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility

• Alphabetized List of Recognition Sequences
• Dam-Dcm and CpG Methylation
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• Why Choose Recombinant Enzymes?

• Double Digest Finder
• Enzyme Finder
• REBASE®

1. What is the difference between AleI (NEB #R0634) and AleI-v2 (NEB #R0685)?
2. When is star activity a concern?
3. I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
4. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
5. Do degenerate recognition sites need to be palindromic?
6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
8. Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0685S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0685L_v1_10032568
• R0685S_v1_10032571
• R0685L_v1_10045831
• R0685S_v1_10045833
• R0685L_v1_10051843
• R0685S_v1_10051025
• R0685L_v1_10058411
• R0685S_v1_10058414
• R0685S_v1_10068136
• R0685L_v1_10068138
• R0685L_v1_10079333
• R0685S_v1_10083529
• R0685S_v1_10091205
• R0685L_v1_10089493
• R0685S_v1_10089494
• R0685L_v1_10100395
• R0685S_v1_10100396
• R0685L_v1_10113058
• R0685S_v1_10122807
• R0685L_v1_10126750
• R0685L_v1_10131721
• R0685S_v1_10131724
• R0685S_v1_10140680
• R0685L_v1_10146935
• R0685S_v1_10146937
• R0685S_v1_10165090
• R0685L_v1_10165089
• R0685L_v1_10183095
• R0685L_v2_10187711
• R0685S_v2_10187712
• R0685S_v2_10212373
• R0685L_v2_10214563
• R0685S_v2_10225267
• R0685L_v2_10227041
• R0685L_v2_10242535
• R0685S_v2_10242863
• R0685L_v2_10257640
• R0685L_v2_10270493
• R0685S_v2_10268084
• R0685L_v2_10282682
• R0685S_v2_10282689
• R0685L_v2_10305703
• R0685S_v2_10305700
• R0685S_v2_10331348
• R0685L_v2_10331347

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• AleI-v2
• rCutSmart™ Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.