High-Fidelity (HF®) Restriction Endonucleases
For over 50 years, New England Biolabs has been developing innovative solutions for molecular biology applications. The respected leader in the field of restriction enzyme biology, NEB has developed a line of High-Fidelity (HF®) Restriction Enzymes. These engineered enzymes have the same specificity as the native enzyme, with the added benefit of reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart™.
Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Optimized performance for a wide range of conditions
High-Fidelity Restriction Enzymes have been engineered by exchanging functional amino acid residues and then screening for optimal mutants that perform under a wide range of conditions. Whether you are setting up digests for 5-15 minutes or overnight, or using varying amounts of enzymes, HF enzymes ensure the performance you need.
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
Choose Product:
Choose Type:
-
Restriction Enzymes at NEB: Over 30 years of Innovation
-
A Modern Day Gene Genie Sir Richard Roberts on Rebase
- DNA Sequences and Maps Tool
- Competitor Cross-Reference Tools
- Enzyme Finder
- Recleavable Filled-in 5' Overhangs
- Alphabetized List of Recognition Sequences
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Recleavable Blunt Ends
- Cleavage Of Supercoiled DNA
- Enzymes with Nonpalindromic Sequences
- Enzymes with Multiple Recognition Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoschizomers
- Restriction Enzyme Troubleshooting Guide
- Restriction of Foreign DNA by E. coli K-12
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Restriction Endonucleases - Survival in a Reaction
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of Restriction Enzymes in PCR Buffers
- Star Activity
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Alteration of Apparent Recognition Specificities Using Methylases
- Dam and Dcm Methylases of E. coli
- Restriction Enzyme Diluent Buffer Compatibility
- Cleavage Close to the End of DNA Fragments
- Double Digests
- Optimizing Restriction Endonuclease Reactions
- Restriction Enzyme Tips
- Heat Inactivation
- Site Preferences
Feature Articles
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. (2013) Massively parallel characterization of restriction endonucleases Nucleic Acids Res ; 41(11), e119. PubMedID: 23605040, DOI: 10.1093/nar/gkt257
- One buffer convenience with no loss of performance
- Reduced star activity eliminates unwanted cleavage
- Time-Saver qualified for 5-15 minute digests, flexible enough to digest overnight.
- Engineered for performance under a wide range of conditions
- Added flexibility without added cost
- Cloning
- Genotyping
- Mutational Analysis
- Mapping
- Probe Preparation
- Sequencing
- Methylation Detection
- Any application that requires high fidelity or flexible reaction setup
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
-
Reduce Star Activity with High-Fidelity Restriction Enzymes
-
Discovering New Restriction Enzymes
-
How do restriction enzymes interact with DNA?
-
Restriction Enzyme Digest Problem: Too Many DNA Bands
-
What is Restriction Enzyme Star Activity?
-
NEB® Restriction Enzyme Double Digest Protocol
-
Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
-
Standard Protocol for Restriction Enzyme Digests
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.
