TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.
Recommended gel percentage range: 1.2-3%
Optimum separation on 2%
Figure 1: TriDye During Electrophoresis
On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change. Figure 2: 100 bp DNA Ladder visualized
100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane.
This product is related to the following categories:
The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
In general, it is recommended to load 1ul per mm of gel lane.
TriDye DNA Ladder is stable for at least 6 months at 25°C
For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.
References
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.
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Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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