Home Markers & Ladders 1 kb Plus DNA Ladder for Safe Stains

1 kb Plus DNA Ladder for Safe Stains

  • Optimized for visualization on GelRed®, GelGreen® and SYBR™ Safe™ precast agarose gels (see Product Notes below for more details)
  • Convenient, ready-to-load format
  • Size range: 100 bp to 10 kb (100 bp and 1 kb ladder in one!)
  • Easy to identify reference bands
  • Suitable for 125 to 250 gel lanes

 

1 kb Plus DNA Ladder for Safe Stains
Blog_ContextualAd
Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
N0559S Not Applicable 1.25 ml $90.00 $90.00
Please enter a quantity for at least one size

  • 1 kb Plus DNA Ladder for Safe Stains is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. It has been optimized for use on GelRed®, GelGreen® and SYBR™ Safe™ precast agarose gels.
     
    1 kb Plus DNA Ladder for Safe Stains consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

    • Recommended gel percentage range: 1-1.4%
    • Optimum separation on 1.2%

    1 kb Plus DNA Ladder for Safe Stains
    1 kb Plus DNA Ladder for Safe Stains visualized on a 1.0% TBE agarose gel precast with GelRed.
 Mass values are for 0.5 µg/lane.
 Suggested Load: 5 to 10 µl/lane.
    This product is related to the following categories:
    DNA Markers & Ladders
    This product can be used in the following applications:
    DNA Analysis

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Bases

      Fragment Mass (ng) bp
      1 20 10002
      2 20 8001
      3 24 6001
      4 20 5001
      5 16 4001
      6 60 3001
      7 20 2017
      8 28 1517
      9 23 1200
      10 61 1000
      11 17 900
      12 15 800
      13 14 700
      14 11 600
      15 62 500/517
      16 25 400
      17 18 300
      18 16 200
      19 30 100

      Effective Size Range

      100bp to 10,002bp

      Storage Buffer

      5 mM Tris-HCl
      5 mM EDTA
      5% Glycerol
      0.002% Xylene Cyanol
      pH 8 @ 25°C

    • Related Products

      Companion Products

    • Product Notes

      1. Preparation of DNA:
        The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
      2. Usage recommendation:
        We recommend loading 5 µl (0.25 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on GelRed® and GelGreen® precast agarose gels. We recommend loading 10 µl (0.5 µg) of 1 kb Plus DNA Ladder for Safe Stains per gel lane on SYBR® Safe precast agarose gels. 
      3. Storage Recommendations:
        1 kb Plus DNA Ladder for Safe Stains is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
      4. Usage Recommendations:
        • The 1 kb Plus DNA Ladder for Safe Stains is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
        • The 1 kb Plus DNA Ladder for Safe Stains might be used on SYBR Green I precast agarose gels but might require further dilution and optimization. We recommend using SYBR Green I as a post-electrophoresis stain for best results.
        • We recommend using SYBR Gold as a post-electrophoresis stain only, as recommended by the dye’s manufacturer.
        • The 1 kb Plus DNA Ladder for Safe Stains is not intended for use on short mini-gels or fast electrophoresis systems. We recommend a minimum of 7 cm of gel length for proper and efficient separation. Best results occur with migration at 5 V/cm (distance between electrodes) for 60 to 90 minutes.
        • Most gel stains run in the opposite direction from that of the migrating DNA. As a result, the bottom of the precast gel might have a lower concentration of stain, which might result in un-uniform staining of the bottom bands. The length of the gel that will contain a lower stain concentration is highly dependent on the migration conditions (gel percentage, voltage, buffer, run time, etc.). This effect can be counteracted by adding some gel stain to the running buffer.

    • References

      1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

  • Protocols, Manuals & Usage

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.

Choose your country

North America

Europe

Asia-Pacific

If you don't see your country above, please visit our international site

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.