Quick CIP

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0525S     -20       Quick CIP M0525SVIAL -20 1 x 0.2 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
M0525L     -20       Quick CIP M0525LVIAL -20 1 x 1 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Features

• Rapid and irreversible heat inactivation eliminates unwanted activity
• Improved storage stability versus native enzyme
• Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
• Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
• Less enzyme required (high specific activity), resulting in a lower cost per reaction
• No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
• Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
• Recombinant for purity, consistency and value

Application Features

• Dephosphorylation 5′ and 3′ ends of DNA and RNA.
• Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
• Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
• Removal of dNTPs in PCR reactions prior to sequencing or SNP analysis.

Companion Products

• M0568 Thermolabile Exonuclease I
• Exonuclease I (E. coli)
• Blunt/TA Ligase Master Mix
• Instant Sticky-end Ligase Master Mix
• Quick Ligation™ Kit
• T4 DNA Ligase
• Exo-CIP™ Rapid PCR Cleanup Kit

1. Quick CIP, as are most alkaline phosphatases, is a Zn₂+ and Mg₂+-dependent enzyme. Our formulation has tightly bound zinc atoms in the active center and does not require supplemental zinc or other additives.
2. Quick CIP is also active in 1X NEBuffers 1.1, 2.1, 3.1 as well as NEBuffers 1, 2, 3 and 4 and NEBuffer for EcoRI.
3. Quick CIP activity is enhanced in the presence of monovalent salts.
4. Quick CIP is inhibited by metal chelators (e.g. EDTA), inorganic phosphate and phosphate analogs.
5. The Quick CIP activity is decreased in the presence of reducing agents (DTT, β-mercaptoethanol).
6. Quick CIP is a homodimer. The molecular weight of the monomer is 56 kDa.

1. Weissig, H. et al (1993). Biochem. J. 290, 503-508.
2. Manes, T (1998). J. Biol. Chem. 273, 23353-23360.

1. Protocol for Dephosphorylation of 5′ ends of DNA using Quick CIP (NEB #M0525)
2. Enzymatic PCR Cleanup Protocol (NEB #M0525)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• Phosphatase Selection Chart

• NEBcloner^®

1. Which alkaline phosphatase, Quick CIP, rSAP or Antarctic Phosphatase works best?
2. The number of colonies that do not contain an insert seems high. How can I tell if Quick CIP worked?
3. What phosphate groups are removed by rSAP, Quick CIP, or Antarctic Phosphatase (AnP)?
4. Does the DNA need to be purified after treatment with Quick CIP?
5. What is the effect of metal chelators, inorganic phosphate and phosphate analogs on Quick CIP activity?
6. What is the effect of monovalent salts on Quick CIP activity?
7. What is the effect of reducing agents on Quick CIP activity?
8. Will Quick CIP, rSAP or Antarctic Phosphatase (AnP) dephosphorylate proteins?
9. Can Quick CIP be heat inactivated?
10. Does the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?
11. Are the alkaline phosphatases active in NEBuffers?
12. Cloning Problem: Too much background in the transformation step.
13. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0525S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0525L_v1_10047913
• M0525S_v1_10047911
• M0525S_v1_10050886
• M0525S_v1_10057020
• M0525L_v1_10057243
• M0525S_v1_10058671
• M0525S_v1_10062172
• M0525L_v1_10061379
• M0525S_v1_10067921
• M0525S_v1_10072416
• M0525S_v1_10080098
• M0525L_v1_10072418
• M0525S_v1_10084302
• M0525L_v1_10091167
• M0525S_v1_10091111
• M0525S_v1_10089450
• M0525L_v1_10089449
• M0525S_v1_10096487
• M0525S_v1_10098690
• M0525L_v1_10102147
• M0525S_v1_10104487
• M0525S_v1_10107424
• M0525S_v1_10117218
• M0525L_v1_10107421
• M0525S_v1_10117841
• M0525S_v1_10121353
• M0525S_v1_10128982
• M0525S_v1_10132820
• M0525L_v1_10139070
• M0525S_v1_10139069
• M0525S_v1_10148966
• M0525L_v1_10152104
• M0525S_v1_10152098
• M0525S_v1_10161214
• M0525L_v1_10168539
• M0525S_v1_10168540
• M0525S_v1_10170246
• M0525S_v1_10175798
• M0525S_v1_10180328
• M0525S_v1_10194852
• M0525L_v1_10195900
• M0525S_v1_10202512
• M0525S_v1_10206285
• M0525S_v1_10220981
• M0525L_v1_10226663
• M0525S_v1_10226664
• M0525L_v1_10238398
• M0525S_v1_10238399
• M0525L_v1_10246929
• M0525S_v1_10246928
• M0525L_v1_10256901
• M0525S_v1_10256899
• M0525L_v1_10267523
• M0525S_v1_10268559
• M0525L_v1_10276406
• M0525S_v1_10279038
• M0525L_v1_10288825
• M0525S_v1_10288827
• M0525S_v1_10300266
• M0525L_v1_10302062
• M0525L_v1_10311353
• M0525S_v1_10311352
• M0525L_v1_10321745
• M0525S_v1_10321744
• M0525L_v1_10331650

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Quick CIP
• rCutSmart™ Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.