T7 DNA Polymerase (unmodified)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0274S     -20       T7 DNA Polymerase (unmodified) M0274SVIAL -20 1 x 0.03 ml 10,000 units/ml   T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
M0274L     -20       T7 DNA Polymerase (unmodified) M0274LVIAL -20 1 x 0.15 ml 10,000 units/ml   T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

Application Features

• Second strand synthesis in site-directed mutagenesis protocols (8).

Companion Products

• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set

Materials Sold Separately

• Recombinant Albumin, Molecular Biology Grade
  
  
  
  
  

1. The high polymerization rate of the enzyme makes long incubations unnecessary.
2. T7 DNA Polymerase is not suitable for DNA sequencing.

1. Hori, K. et al. (1979). J. Biol. Chem.. 254, 11598-11604.
2. Engler, M.J. et al. (1983). J. Biol. Chem.. 258, 11165-11173.
3. Nordstrom, B. et al. (1981). J. Biol. Chem.. 256, 3112-3117.
4. Studier, F.W. et al. (1990). Goeddel, D.V.(Ed.), In Methods Enzymol.. 185, 60-89. San Diego: Academic Press.
5. Grippo, P. and Richardson, C.C. (1971). J. Biol. Chem. 246, 6867-6873.
6. Modrich, P. and Richardson, C.C. (1975). J. Biol. Chem. 250, 5515-5522.
7. Adler, S. and Modrich, P. (1979). J. Biol. Chem. 254, 11605-11614.
8. Bebenek, K. and Kunkel, T.A. (1989). Nucl. Acids Res.. 17, 5408.
9. Mattila, P., et al. (1991). Nucleic Acids Res.. 19, 4967-4973.

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• DNA Polymerase Selection Chart

1. Which DNA polymerase can I use to blunt DNA?
2. Can I use a DNA polymerase to fill in 3' overhangs or remove 5' overhangs?
3. How can I heat inactivate this DNA polymerase?
4. Can this DNA polymerase be used in labeling and partial fill reactions?
5. What are the main causes of blunting reaction failure?
6. Can this DNA polymerase be used in other NEBuffers for filling in or blunting overhangs?
7. Does NEB carry modified T7 Polymerase?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0274S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0274S_L_v1_0111508
• M0274S_L_v1_0111603
• M0274S_L_v1_0111609
• M0274S_L_v1_0111703
• M0274S_L_v1_0111803
• M0274L_v1_10012703
• M0274S_v1_10021765
• M0274L_v1_10021767
• M0274L_v1_10039918
• M0274S_v1_10040917
• M0274L_v1_10057374
• M0274S_v1_10059218
• M0274L_v1_10075649
• M0274L_v1_10081788
• M0274S_v1_10091672
• M0274L_v1_10091094
• M0274S_v1_10107595
• M0274S_v1_10114913
• M0274S_v1_10123097
• M0274L_v1_10131164
• M0274S_v1_10135910
• M0274L_v1_10139934
• M0274L_v1_10152153
• M0274S_v1_10161568
• M0274L_v1_10161623
• M0274S_v1_10163746
• M0274S_v1_10165610
• M0274L_v1_10167009
• M0274L_v1_10174179
• M0274S_v1_10179183
• M0274L_v1_10180393
• M0274S_v1_10204170
• M0274S_v1_10215598
• M0274L_v1_10215709
• M0274S_v1_10229465
• M0274L_v1_10231233
• M0274L_v1_10239522
• M0274L_v1_10253683
• M0274S_v1_10267721
• M0274L_v1_10272282
• M0274L_v1_10279062
• M0274S_v1_10290035
• M0274L_v1_10298685
• M0274S_v1_10300310
• M0274S_v1_10313551
• M0274L_v1_10333081
• M0274S_v1_10328062
• M0274S_v1_10338160

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T7 DNA Polymerase (unmodified)
• T7 DNA Polymerase Reaction Buffer
• Recombinant Albumin, Molecular Biology Grade

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

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