NEBNext® Ultra™ Ligation Module

This product was discontinued on December 15, 2025. An alternative product is the NEBNext® Ultra™ II Ligation Module  (NEB #E7595L).

  • Catalog # E7445 was discontinued on December 15, 2025

This module is part of the original Ultra™ workflow, and is optimized for use with the NEBNext® Ultra End Repair/dA-Tailing Module (NEB #E7442), for Illumina®-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol. However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg), and a NEBNext Ultra II Ligation Module (NEB #E7595) is available.

Storage Temperature: -20°C

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