Immobilized T4 DNA Ligase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0569S     -20       Immobilized T4 DNA Ligase M0569SVIAL -20 1 x 0.11 ml 10 mg/ml   T4 DNA Ligase Reaction Buffer B0202SVIAL -20 1 x 1 ml 10 X

Companion Products

• Nuclease-free Water

1. ATP is an essential cofactor for the reaction. This contrasts with E. coli DNA ligase which requires NAD.
2. If IM T4 DNA Ligase is less than 10% of the total reaction volume, set-up reaction in the following order: H2O, buffer, DNA and finally enzyme.
3. If IM T4 DNA Ligase is more than 10% of the total reaction volume, we recommend setting up the reaction as follows:  Prepare the substrate in 1X reaction buffer.  Add IM T4 DNA Ligase into a fresh tube.  Put the tube on a magnet for 3 minutes to pellet the beads.  Then carefully remove the supernatant from the bead pellet and add the substrate mixture to the bead pellet. (Do not remove the supernatant until you are ready to assemble the reaction, as the beads should not be allowed to dry out.) 
4. If incubation is longer than 30 minutes, agitation will be required to ensure beads remain in suspension.

1. Engler, M.J. and Richardson, C.C. (1982). P.D. Boyer(Ed.), 5, 3. San Diego: Academic Press.

1. Protocol for Ligation of Fragments with Cohesive Ends using Immobilized T4 DNA Ligase (NEB #M0569)
2. Protocol for Ligation of Fragments with Splinted or Cohesive Ends using Immobilized T4 DNA Ligase (NEB #M0569)

• Generating Oligonucleotide Probes using Immobilized T4 DNA Ligase minimizes sample loss and saves time

• DNA Ligase Selection Chart
• Properties of DNA and RNA Ligases
• Substrate-based Ligase Selection Chart
• T4 DNA Ligase Products Table

• NEBioCalculator®

1. How should Immobilized T4 DNA Ligase (NEB #M0569) be stored and handled?
2. How should I mix or resuspend Immobilized T4 DNA Ligase (NEB #M0569) beads prior to use?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0569S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0569S_v1_10098368
• M0569S_v1_10143820
• M0569S_v1_10191243
• M0569S_v1_10241822
• M0569S_v1_10272618
• M0569S_v1_10283316
• M0569S_v1_10303899

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Immobilized T4 DNA Ligase
• T4 DNA Ligase Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.