Home RNA Synthesis and Modification m7G(5')ppp(5')A RNA Cap Structure Analog

m7G(5')ppp(5')A RNA Cap Structure Analog

Co-transcriptional capping with T7 RNA polymerase from the phi2.5 promoter that contains an A at the transcription initiation site.

  • Synthesis of m7G capped RNA for in vitro splicing assays.
  • Synthesis of m7G capped RNA for transfection or microinjection.

Featured Video

RNase_Contamination_Anim_720x405

Avoiding RNase Contamination

Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
S1405S Not Applicable 1 µmol $197.00 $197.00
S1405L Not Applicable 5 µmol $783.00 $783.00
Please enter a quantity for at least one size
Need a custom/large volume order? Contact us
  • Description: The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

    A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (7). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5´)ppp(5´)G (6).

    Figure 1: Structure of m7G(5´)ppp(5´)A RNA Cap Structure Analog (NEB #S1405)

    Structure of m7G(5')ppp(5')A RNA Cap Structure Analog

    Sequence

    7mG(5')ppp(5')A
    This product is related to the following categories:
    RNA Capping

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Supplied As

      Dried Sodium Salt

      Suggested Working Concentration

      10mM

      Recommended Resuspension Volume

      100 μl

    • Product Notes

      1. Addition of 100 µl of water gives approximately a 10 mM solution.

    • References

      1. Shatkin, A.J. (1978). Cell. 9, 645-653.
      2. Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
      3. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
      4. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
      5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
      6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
      7. Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-6363.
      8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

  • Quality, Safety & Legal

Featured Videos

View Video Library

Other Products You May Be Interested In

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.

Choose your country

North America

Europe

Asia-Pacific

If you don't see your country above, please visit our international site

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.