Home Reagents and Molecular Biology Products Protein Expression Cell-Free Protein Expression PURExpress® In Vitro Protein Synthesis Tailored PURExpress® Kits for Flexible Cell-Free Protein Expression

Tailored PURExpress® Kits for Flexible Cell-Free Protein Expression

Return to PURExpress® In Vitro Protein Synthesis

Take control with more kit options that meet your exact needs

Diagram of tailored PURExpress® Kits for cell-free protein expressionNEB® now offers a new suite of customized PURExpress® in vitro protein synthesis kits through our Customized Solutions channel. Designed for researchers seeking greater control over cell-free protein synthesis (CFPS), these pre-configured kits feature specific components of the PURExpress system selectively omitted, giving you the freedom to experiment with your own additions or modifications. Each kit is built to order with a defined configuration, making it easier to select the right solution for your needs without starting from scratch.

These kits are ready-to-order through our Customized Solutions Team.
 
Note: a minimum order quantity may be required, and lead times will vary.

 

Contact us to order

 


 Custom PURExpress Δ T7 RNA Polymerase Kit Custom PURExpress Δ (aa, tRNA, RF123) Kit Custom PURExpress Δ (aa, tRNA, RF123, Ribosomes) Kit Custom PURExpress Δ (RF123, Ribosomes) Kit Custom PURExpress Δ (CTP, UTP, RF123, T7 RNAP) Kit
 





 Common applications Enables use of alternative RNA polymerases mRNA Display & Ribosome Display mRNA Display & Ribosome Display Ribosome Display & translation studies Translation studies; enables use of alternative RNA polymerases
Custom Kit Part Number
 E6810B-001 E6844B-001 E6845B-001 E6851B-001 E6852B-001






Reactions Per Kit  10 10 10 10 10
Catalog Kit(s) Most Similar PURExpress In Vitro Protein Synthesis Kit (NEB #E6800) PURExpress Δ (aa, tRNA) Kit (NEB #E6840) + PURExpress Δ RF123 Kit (NEB #E6850) PURExpress Δ Ribosome Kit (NEB #E3313) + PURExpress Δ (aa, tRNA) Kit (NEB #E6840) + PURExpress Δ RF123 Kit (NEB #E6850) PURExpress Δ Ribosome Kit (NEB #E3313) + PURExpress Δ RF123 Kit (NEB #E6850) PURExpress Δ RF123 Kit (NEB #E6850)
Component Name Component Part Number




Component Volume (mL)
PURExpress Control DHFR plasmid N0424B-001 ✓ ✓ ✓ ✓ ✓ 0.010
PURExpress Solution A 
(-aa, -tRNA)		 B6841B-001
✓ ✓

0.050
Amino Acid Mix N6843B-001
✓ ✓

0.025
tRNA Mix (available in catalog as NEB #N6845) N6842B-001 / N6845
✓ ✓

0.025
PURExpress Solution B 
(-RF123)		 P6854B-001
✓


0.075
PURExpress Factor Mix 
(-RF123)		 P0825B-001

✓ ✓
0.030
PURExpress Ribosomes 
(RF free)		 P0824B-001

✓ ✓
0.045
PURExpress RF1 P6851B-001
✓ ✓ ✓ ✓ 0.010
PURExpress RF2 P6852B-001
✓ ✓ ✓ ✓ 0.010
PURExpress RF3 P6853B-001
✓ ✓ ✓ ✓ 0.010
PURExpress Solution A B0228B-001 ✓

✓
0.100
PURExpress Solution B 
(-T7 RNAP)		 P0775B-001 ✓



0.075
PURExpress control T7 RNAP P0874B-001 ✓


✓ 0.010
PURExpress Solution A 
(-CTP, UTP)		 B0234B-001



✓ 0.100
PURExpress control CTP, UTP mix N0250B-001



✓ 0.010
PURExpress Solution B 
(-RF123, -T7 RNAP)		 P6863B-001



✓ 0.075

 

Related Resources:

Other Product Categories
Experts in protein expression and purification
Nucleic Acid Purification
NEBuilder® HiFi DNA Assembly
Push the Limits of Golden Gate Assembly
 App Notes
Resource widget icon representing application note Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads
Resource widget icon representing application note  Cell-free Expression and Rapid Analysis of Single-Chain Antibody Fragments
Resource widget icon representing application note
 Scaling down to scale up Miniaturizing cell free protein synthesis reactions with the Echo 525 Acoustic Liquid Handler

Learn about PURExpress® In Vitro Protein Synthesis