E. coli RNA Polymerase, Holoenzyme
E. coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters.
- RNA synthesis from E. coli promoter
- Transcription initiation studies
- In vitro translation with PURExpress®
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Product Information
E. coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters.
E. coli RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω. The enzyme is free of sigma factor and does not recognize any specific bacterial or phage DNA promoters. The enzyme retains the ability to transcribe RNA from nonspecific initiation sequences. Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters. The core enzyme has a molecular weight of approximately 400 kDa.Product Source
E. coli RNA Polymerase, Holoenzyme is isolated from E. coli strain BL21. The sigma factor 70 is purified from an E. coli strain carrying the cloned gene for sigma factor 70.- This product is related to the following categories:
- RNA Modification,
- Nucleotide Solutions for RNA,
- RNA Synthesis In vitro Transcription (IVT)
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