Exonuclease VIII, truncated

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0545S     -20       Exonuclease VIII, truncated M0545SVIAL -20 1 x 0.1 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X

Application Features

•    Degradation of linear dsDNA while maintaining double stranded circular DNA.

1. Chang, et al. (2001). J. Biol. Chem.. 46004–46010.

1. Protocol for Exonuclease VIII, truncated (M0545)

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

1. How does Exonuclease VIII, truncated differ from Lambda Exonuclease (NEB# M0262)?
2. Will Exonuclease VIII, truncated cleave supercoiled dsDNA or covalently close circular form dsDNA?
3. How does Exonuclease VIII, truncated differ from Exonuclease III (NEB# M0206)?
4. Can DNA be blunted using Exonuclease VIII, truncated?
5. What is the activity of Exonuclease VIII, truncated in these common buffers?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0545S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0545S_v1_10009909
• M0545L_v1_10009911
• M0545S_v1_10024968
• M0545L_v1_10034575
• M0545S_v1_10042369
• M0545L_v1_10051173
• M0545S_v1_10059606
• M0545L_v1_10059609
• M0545S_v1_10089251
• M0545S_v1_10099162
• M0545S_v1_10102105
• M0545S_v1_10132863
• M0545S_v1_10163731
• M0545S_v1_10182560
• M0545S_v1_10208837
• M0545S_L_v1_0031708
• M0545S_v1_10220983
• M0545S_v1_10246924
• M0545S_v1_10262427
• M0545S_v1_10279524
• M0545S_v1_10316615
• M0545S_v1_10328168

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Exonuclease VIII, truncated
• NEBuffer™ 4

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.