T4 Phage β-glucosyltransferase (T4-BGT)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0357S     -20       T4 Phage β-glucosyltransferase (T4-BGT) M0357SVIAL -20 1 x 0.05 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X   Uridine Diphosphate Glucose S2200SVIAL -20 1 x 0.25 ml 2 mM
M0357L     -20       T4 Phage β-glucosyltransferase (T4-BGT) M0357LVIAL -20 1 x 0.25 ml 10,000 units/ml   NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X   Uridine Diphosphate Glucose S2200SVIAL -20 1 x 0.25 ml 2 mM

Materials Sold Separately

• NEBuffer 4

1. Josse, J. and Kornberg, A. (1962). J. Biol.Chem.. 237, 1968-1976.
2. Tomaschewski, J. et al. (1985). Nucleic Acids Res. 13, 7551-7568.
3. McNicol, L. A. et al. (1973). J. Mol. Biol. 15, 76,, 285-301.
4. Szwagierczak, A. et al. (2010). Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucleic Acids Res. 38, e181.

1. Protocol for Glucosylation and digestion of Genomic DNA using AbaSI (#R0665)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

1. Will T4 Phage β-glucosyltransferase (T4-BGT) work in rCutSmart Buffer?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0357S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0357L_v1_10010996
• M0357S_v1_10010998
• M0357L_v1_10016771
• M0357L_v1_10025417
• M0357S_v1_10033820
• M0357L_v1_10033822
• M0357S_v1_10046686
• M0357L_v1_10049498
• M0357L_v1_10062034
• M0357S_v1_10062719
• M0357L_v1_10064357
• M0357L_v1_10088103
• M0357S_v1_10094870
• M0357S_v1_10111270
• M0357L_v1_10115395
• M0357S_v1_10126619
• M0357L_v1_10134498
• M0357L_v1_10150167
• M0357L_v1_10151099
• M0357S_v1_10157693
• M0357S_v1_10166704
• M0357S_v1_10208788
• M0357L_v1_10203068
• M0357S_L_v1_0051801
• M0357S_v1_10225261
• M0357L_v1_10245626
• M0357S_v1_10274335
• M0357L_v1_10284247
• M0357S_v1_10298982
• M0357L_v1_10308400
• M0357S_v1_10316444

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T4 Phage β-glucosyltransferase (T4-BGT)
• NEBuffer™ 4
• Uridine Diphosphate Glucose

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.