Afu Uracil-DNA Glycosylase (UDG)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0279S     -20       Afu Uracil-DNA Glycosylase (UDG) M0279SVIAL -20 1 x 0.1 ml 2,000 units/ml   ThermoPol® II (Mg-free) Reaction Buffer B9005SVIAL -20 1 x 1.5 ml 10 X

Materials Sold Separately

• ThermoPol® II (Mg-free) Reaction Buffer Pack

1. Afu UDG retains 50% activity in the presence of 150 mM NaCl. Afu UDG retains less than 1% activity after boiling for 30 minutes in standard reaction conditions.
2. Under standard reaction conditions, uracil glycosylase inhibitor (UGI) does not inhibit Afu UDG.

1. Sandigursky, M. and Franklin, W. A. (1999). CurrentBiology. 9,
2. Sandigursky, M. and Franklin, W. A. (2000). J. Biol.Chem.. 275,

• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. Will Afu UDG work in Thermopol Buffer?
2. What is the molecular weight of Afu UDG?
3. Is Afu UDG a tagged protein?
4. I see that Afu UDG works optimally at 65°C. Do you have another glycosylase that works at lower temperatures?
5. Does the Uracil Glycosylase inhibitor (UGI) inhibit Afu UDG?
6. Does Afu UDG release uracil from ss and dsDNA?
7. Does Afu UDG cut RNA?
8. Are there any specific recommendations for the use of UDG on single stranded DNA?
9. Will Afu UDG work in Fpg buffer?
10. What is the activity of WarmStart Afu Uracil-DNA Glycosylase (UDG) in NEBuffers?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0279S_L_v3
• M0279S_v4

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0279S_L_v2_0031501
• M0279S_L_v1_0031507
• M0279S_L_v2_0031512
• M0279S_L_v3_0031608
• M0279S_L_v3_0031705
• M0279S_L_v3_0031707
• M0279S_L_v3_0031711
• M0279S_L_v3_0031801
• M0279S_v3_10010391
• M0279L_v3_10010393
• M0279L_v3_10027915
• M0279L_v3_10033386
• M0279S_v3_10027917
• M0279L_v3_10034346
• M0279S_v3_10047840
• M0279L_v3_10055584
• M0279L_v3_10068174
• M0279S_v3_10068175
• M0279S_v3_10097046
• M0279S_v3_10112350
• M0279S_v3_10137307
• M0279S_v3_10173156
• M0279S_v4_10226501
• M0279S_v4_10246066
• M0279S_v4_10281664
• M0279S_v4_10300284
• M0279S_v4_10328063

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Afu Uracil-DNA Glycosylase (UDG)
• ThermoPol^® II (Mg-free) Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.