Home RNA Synthesis and Modification RNase If

RNase If
NEB3 recombinant Gray property icon for incubation temperature 37° 70 Heat

RNase If eliminates single-stranded RNA from DNA and protein preparations 

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNA endonucleases and exonucleases 
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.

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BehindthePaper_Ecoli

Behind the Paper: E.coli RNase I exhibits a Ca2+-dependent double-stranded RNase activity 

Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M0243S 50,000 units/ml 5,000 units $85.00 $85.00
M0243L 50,000 units/ml 25,000 units $337.00 $337.00
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  • Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
    and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA in the absence of calcium (2). RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.

    Product Source

    An E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).
    This product is related to the following categories:
    RNases,
    Epitranscriptome Analysis

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 µl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.

      Reaction Conditions

      1X NEBuffer™ 3
      Incubate at 37°C

      1X NEBuffer™ 3
      100 mM NaCl
      50 mM Tris-HCl
      10 mM MgCl2
      1 mM DTT
      (pH 7.9 @ 25°C)

      Storage Buffer

      10 mM Tris-HCl
      100 mM NaCl
      1 mM DTT
      0.5 mM EDTA
      50% Glycerol
      pH 8 @ 25°C

      Heat Inactivation

      70°C for 20 minutes

    • Advantages and Features

      Application Features

      • Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
      • Used in ribonuclease protection assays

    • Related Products

      Materials Sold Separately

    • Product Notes

      1. RNase If does not degrade DNA. It preferentially cleaves single-stranded RNA over double-stranded RNA. Digestion of dsRNA can be enhanced by the addition of Ca²⁺. RNase If can also degrade the RNA strand of RNA:DNA hybrid duplexes.

    • References

      1. Spahr, P.F. and Hollingworth, B.R. (1961). J. Biol. Chem.. 236, 823-831.
      2. Grünberg S, Coxam B, Chen TH, Dai N, Saleh L, Corrêa IR, Nichols NM, Yigit E. (2021). Nucleic Acids Res.. May 21;49(9), 5265-5277.
      3. Meador, J. III and Kennell, D. (1990). Gene. 95, 1-7.
      4. Meador, J. III, Cannon, B., Cannistraro, V.J. and Kennell, D. (1990). Eur. J. Biochem.. 187, 549-553.

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