Human DNA (cytosine-5) Methyltransferase (Dnmt1)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0230S     -20       Human DNA (cytosine-5) Methyltransferase (Dnmt1) M0230SVIAL -20 1 x 0.025 ml 2,000 units/ml   Dnmt1 Reaction Buffer B0230SVIAL -20 1 x 1.5 ml 10 X   Purified BSA B9001SVIAL -20 1 x 0.25 ml 10 mg/ml   S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM
M0230L     -20       Human DNA (cytosine-5) Methyltransferase (Dnmt1) M0230LVIAL -20 1 x 0.125 ml 2,000 units/ml   Dnmt1 Reaction Buffer B0230SVIAL -20 1 x 1.5 ml 10 X   Purified BSA B9001SVIAL -20 1 x 0.25 ml 10 mg/ml   S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Materials Sold Separately

• Purified BSA
• S-adenosylmethionine (SAM)

1. For DNA modification and protection applications, M.SssI (NEB #M0226) is preferred because it efficiently methylates both unmethylated and hemimethylated DNA substrates.

1. Pradhan, S. et al. (1999). J. Biol. Chem. . 274, 33002-33010.
2. Bacolla, A. et al. (1999). J. Biol. Chem. . 274, 33011-33019.
3. Schmutte, C. et al. (1998). Biol. Chem. . 379, 377-388.
4. Laird, P.W. et al. (1995). Cell. 81, 197-205.
5. Li, E. et al. (1992). Cell. 12, 915-926.
6. Leonhardt, H. et al. (1992). Cell. 71, 865-873.
7. Yen, R.W. et al. (1992). Nucl. Acids. Res. . 20, 2287-2291.

1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
2. What source of tritiated SAM is recommended for use with Dnmt1?
3. What should be considered if the methylation using Dnmt1 is not going to completion?
4. How does Dnmt1 differ from SssI methylase?
5. Can Dnmt1 be heat inactivated?
6. Will any NEB methyltransferases (methylases) work on RNA?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0230S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0230S_v1_10009673
• M0230L_v1_10028052
• M0230S_v1_10028046
• M0230L_v1_10035552
• M0230S_v1_10033941
• M0230L_v1_10042377
• M0230S_v1_10042375
• M0230L_v1_10053616
• M0230S_v1_10053614
• M0230S_v1_10059149
• M0230L_v1_10059151
• M0230L_v1_10067941
• M0230S_v1_10067944
• M0230L_v1_10083648
• M0230S_v1_10083651
• M0230L_v1_10086920
• M0230S_v1_10094047
• M0230L_v1_10100068
• M0230S_v1_10100065
• M0230L_v1_10117330
• M0230S_L_v1_0391710
• M0230S_L_v1_0391712

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Human DNA (cytosine-5) Methyltransferase (Dnmt1)
• Dnmt1 Reaction Buffer
• Purified BSA
• S-adenosylmethionine (SAM)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.