TaqI Methyltransferase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
NEB # Stored at (°C) M0219S -20 Component Name Component # Stored at (°C) Amount Concentration TaqI Methyltransferase M0219SVIAL -20 1 x 0.1 ml 10,000 units/ml rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Materials Sold Separately

• NEBuffer 4
• Purified BSA
• S-adenosylmethionine (SAM)

1. TaqI Methyltransferase gives 25% activity at 37°C.

1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

1. Recommended Protocol for 3H labeling of DNA
2. Protocol for Methylation of Genomic DNA Using Methyltransferases

1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
2. What should be considered if the methylation is not going to completion?
3. What is the activity of TaqI Methyltransferase at 37°C?
4. What is the activity of TaqI Methyltransferase at 50°C?
5. Does TaqI Methyltransferase require MgCl₂?
6. Does TaqI Methyltransferase require BSA?
7. What is the activity of TaqI Methyltransferase in other buffers?
8. What is the molecular weight of TaqI Methyltransferase?
9. Is TaqI Methyltransferase used in any special techniques?
10. Will any NEB methyltransferases (methylases) work on RNA?
11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0219S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0219S_v1_10041616
• M0219S_v1_10046909
• M0219S_v1_10051832
• M0219S_v1_10056210
• M0219S_v1_10067098
• M0219S_v1_10092121
• M0219S_v1_10091499
• M0219S_v1_10115352
• M0219S_v1_10146856
• M0219S_v1_10163792
• M0219S_v1_10210687
• M0219S_v1_0051803
• M0219S_v1_10225044
• M0219S_v1_10237018
• M0219S_v2_10281979
• M0219S_v2_10309152
• M0219S_v2_10336149
• M0219S_v2_10352598

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• TaqI Methyltransferase
• rCutSmart™ Buffer
• S-adenosylmethionine (SAM)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.