NEBNext^® Companion Module v2 for Oxford Nanopore Technologies^® Ligation Sequencing

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7672S     -20       NEBNext® FFPE DNA Repair Buffer v2 E7673AVIAL -20 1 x 0.168 ml Not Applicable   NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not Applicable   NEBNext® Ultra™ II End Prep Enzyme Mix E7184AVIAL -20 1 x 0.072 ml Not Applicable   Salt-T4® DNA Ligase E7674AVIAL -20 1 x 0.24 ml Not Applicable
E7672L     -20       NEBNext® FFPE DNA Repair Buffer v2 E7673AAVIAL -20 1 x 0.672 ml Not Applicable   NEBNext® FFPE DNA Repair Mix E7182AAVIAL -20 1 x 0.192 ml Not Applicable   NEBNext® Ultra™ II End Prep Enzyme Mix E7184AAVIAL -20 1 x 0.288 ml Not Applicable   Salt-T4® DNA Ligase E7674AAVIAL -20 1 x 0.96 ml Not Applicable

• Usage guidelines for reagents included in the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672)

1. What is the difference between NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672) & NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7180)?
2. Is the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing the best choice if I am performing native barcoding ONT sequencing?
3. Which protocol should be followed when using the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7672S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7672L_v1_10249029
• E7672S_v1_10254718
• E7672S_v1_10260132
• E7672S_v1_10249024
• E7672L_v1_10266072
• E7672S_v1_10266074
• E7672S_v1_10266284
• E7672L_v1_10262607
• E7672S_v1_10270176
• E7672S_v1_10262604
• E7672L_v1_10268968
• E7672S_v1_10279699
• E7672S_v1_10268963
• E7672L_v1_10286013
• E7672S_v1_10298150
• E7672S_v1_10286010
• E7672L_v1_10311404
• E7672S_v1_10317651
• E7672L_v1_10301294
• E7672S_v1_10301291
• E7672L_v1_10323772
• E7672S_v1_10333014
• E7672S_v1_10338920

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext^® FFPE DNA Repair Mix
• NEBNext^® Ultra™ II End Prep Enzyme Mix

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.