SNAP-tag Substrates
NEB offers a large selection of fluorescent labels (substrates) for SNAP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. Substrates label the SNAP-tag® without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell®) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface®) are specific for fusion proteins expressed on the cell surface only.
SNAP-tag®, SNAP-Cell® and SNAP-Surface® are registered trademarks of New England Biolabs, Inc.
NEB offers a large selection of fluorescent labels (substrates) for SNAP-tag fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. Substrates label the SNAP-tag® without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell®) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface®) are specific for fusion proteins expressed on the cell surface only.
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Choose Type:
- Cellular Labeling (S9103)
- Protocol for Cellular Labeling with SNAP-Cell® Oregon Green® (NEB #S9104)
- SNAP-Cell® TMR-Star Cellular Labeling (NEB #S9105)
- Cellular Labeling (S9107)
- Cellular Labeling (S9109)
- Cellular Labeling (S9110)
- Cellular Labeling (S9112)
- Cellular Labeling (S9124)
- Cellular Labeling (S9129)
- Cellular Labeling (S9132)
- Cellular Labeling (S9134)
- Cellular Labeling with SNAP-Surface® Alexa Fluor® 647 (NEB #S9136)
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Cloning of CLIP-tag Fusions in pCLIPf (N9215)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of CLIP-tag Fusions (N9215)
- Expression of MCP Fusions (N9317)
- Expression of MCP-GPI (N9320)
- Labeling Mammalian Cell Lysates (S9147)
- Labeling of Proteins in vitro (S9110)
- Labeling of Proteins in vitro (S9103)
- Labeling of Proteins in vitro (S9104)
- Labeling of Proteins in vitro (S9105)
- Labeling of Proteins in vitro (S9106)
- Labeling of Proteins in vitro (S9107)
- Labeling of Proteins in vitro (S9109)
- Labeling of Proteins in vitro (S9143)
- Labeling Proteins in vitro (S9112)
- Labeling Proteins in vitro (S9124)
- Labeling Proteins in vitro (S9129)
- Labeling Proteins in vitro (S9132)
- Labeling Proteins in vitro (S9134)
- Labeling Proteins in vitro (S9136)
- Labeling Proteins in vitro (S9147)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9150)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
- Labeling SNAP-tag Purified Protein In Vitro (NEB #P9312)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Cloning of SNAP-tag Fusions in pSNAP-tag(T7)-2 (N9181)
- Expression of SNAP-tag Fusions (N9181)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
- Use with SNAP-Surface substrates (S9143)
- Reaction Conditions for Chemical Coupling (S9148)
- Reaction Conditions for Chemical Coupling (S9153)
- Use SNAP-Capture Magnetic Beads (S9145)
- Use SNAP-Capture Pull Down Resin (S9144)
- Expression of CLIPf-NK1R (N9216)
- Expression of CLIPf-Cox8A (N9217)
- Expression of CLIPf-H2B (N9218)
- Reaction Conditions for Chemical coupling (S9150)
- Reaction Conditions for Chemical Coupling (S9151)
- Use with SNAP-Cell Substrates (S9106)
- Cellular Labeling (S9159)
- Labeling of Proteins in vitro (S9159)
- SNAP-Cell® 647-SiR Cellular Labeling (NEB #S9102)
- Labeling of Proteins in vitro (S9102)
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SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
- La Clair, J.J. et al. (2004) Manipulation of carrier proteins in antibiotic biosynthesis Chem Biol; 11, 195-201 . PubMedID: 15123281
- George N. et al. (2004) Specific labeling of cell surface proteins with chemically diverse compounds J Am Chem Soc; 126, 8896-8897 . PubMedID: 15264811
- Prummer M. et al. (2006) Post-translational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells Chembiochem; 7, 908-911 . PubMedID: 16607667
- Jacquier V. et al. (2006) Visualizing receptor trafficking in living Proc Natl Acad Sci U S A; 103, 14325-14330 . PubMedID: 16980412
- Meyer B.H. et al. (2006) Covalent labeling of cell-surface proteins for in vivo FRET studies FEBS Lett; 580, 1654-1658 . PubMedID: 16497304
- Meyer B.H. et al. (2006) FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells Proc Natl Acad Sci U S A; 103, 2138-43 . PubMedID: 16461466
- Vivero-Pol L. et al. (2005) Multicolor imaging of cell surface proteins J Am Chem Soc; 127, 12770-12771 . PubMedID: 16159249
- Yin J. et al. (2005) Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling Chem Biol; 12, 999-1006 . PubMedID: 16183024
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Cravatt B.F. (2005) Live chemical reports from the cell surface Chem Biol; 12, 954-956 . PubMedID: 16183017
- Yin J. et al. (2005) Labeling proteins with small molecules by site-specific posttranslational modification J Am Chem Soc; 126 , 7754-7755 . PubMedID: 15212504
- Zelman-Femiak, M. et al. (2010) Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies Biotechniques; 49, 2. PubMedID: 20701592
- Mosiewicz, K. A. et al. (2010) Phosphopantetheinyl Transferase-Catalyzed Formation of Bioactive Hydrogels for Tissue Engineering J Am Chem Soc; 132, 5972-5974 . PubMedID: 20373804
- Waichman S. et al. (2010) Functional Immobilization and Patterning of Proteins by an Enzymatic Transfer Reaction Anal Chem; 82 , 1478-1485 . PubMedID: 20092261
- Generosi J. et al. (2008) Photobleaching-free infrared near-field microscopy localizes molecules in neurons J Appl Physiol; 104, 106102-1/3.
- Kropf M. et al. (2008) Subunit-specific surface mobility of differentially labeled AMPA receptor subunits Eur J Cell Biol; 87, 763-778 . PubMedID: 18547676
- Generosi J. et al. (2008) AMPA receptor imaging by infrared scanning near-field optical microscopy Physica Status Solidi C: Current Topics in Solid State Physics; 5, 2641-2644 .
- Sunbul M. et al. (2008) Enzyme catalyzed site-specific protein labeling and cell imaging with quantum dots Chem Commun; 5927-5929 . PubMedID: 19030541
- Zhou Z. et al. (2007) Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases ACS Chem Biol; 2, 337-346 . PubMedID: 17465518
- Liu E and Bruner S. D. (2007) Rational manipulation of carrier-domain geometry in nonribosomal peptide synthetases Chembiochem; 8, 617 - 621 . PubMedID: 17335097
- Gralle M. et al. (2009) Neuroprotective secreted amyloid precursor protein acts by disrupting amyloid precursor protein dimers J Biol Chem; 284, 15016-15025 . PubMedID: 19336403
- Neugart F. et al. (2009) Detection of ligand-induced CNTF receptor dimers in living cells by fluorescence cross correlation spectroscopy Biochim Biophys Acta; 1788 , 1890-1900 . PubMedID: 19482006
- Eggeling C. et al. (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell Nature; 457 , 1159-1163. PubMedID: 19098897
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