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DNase Selection Chart

NEB’s DNase Selection Chart will assist in choosing the best enzyme for your application

Application DNase I
NEB #M0303		 DNase I-XT
NEB #M0570		 Duplex DNase
NEB #M7635		 NEBExpress® Salt-Active Nuclease
NEB #M0764
Removal of contaminating genomic DNA from RNA samples +++ +++ ++ —
Removal of DNA templates from in vitro transcription (IVT) reactions ++ +++ + —
Removal of contaminating gDNA prior to RT-qPCR ++ +++ ++ —
PCR decontamination — — +++ —
Specific degradation of dsDNA in presence of ssDNA — — +++ —
Specific cleavage of DNA strand of DNA:RNA hybrid duplex — — +++ —
Nucleic acid removal for purification of recombinant proteins, small molecules, and viruses; and for reducing viscosity in lysates — — — +++


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