α1-2,4,6 Fucosidase O Typical Reaction Conditions
1. Combine 100 nM of oligosaccharide and H2O (if necessary) to make a 9 μl total reaction volume.
2. Add 1 μl of 10X GlycoBuffer 1 to make a 10 μl total reaction volume.
3. Add 1 μl of α1-2,4,6 Fucosidase O.
4. Incubate at 37°C for 1 hour
Note: removal of α1-2 and α1-4 fucose residues may require more enzyme and longer incubation times.