EcoGII Methyltransferase Protocol (M0603)
COMPONENT | 50 µl REACTION |
Nuclease-free Water | to 50 µl |
CutSmart Buffer (10X) | 5 µl (1X) |
S-adenosylmethionine* |
5 µl (160 µM) |
DNA | up to 1 µg |
Methyltransferase |
1 µl (5 units) |
2. Incubate at 37°C for 60 minutes.
The amount of methylation can be optimized by varying amount of substrate, amount of enzyme and/or incubation time.