Electrocompetent Cells Transformation Protocol
- Thaw electrocompetent cells on ice.
- Transfer 50 μl of electrocompetent cells to a pre-chilled electroporation cuvette with 1 mm gap.
- Add 1 μ l of the assembly product to electrocompetent cells.
- Mix gently by pipetting up and down.
- Once DNA is added to the cells, electroporation can be carried out immediately. It is not necessary to incubate DNA with cells.
- Add 950 μl of room-temperature SOC media to the cuvette immediately after electroporation.
- Place the tube at 37°C for 60 minutes. Shake vigorously (250 rpm) or rotate.
- Warm selection plates to 37°C.
- Spread 100 μl of the cells onto the plates.
- Incubate overnight at 37°C.