Sample preparation using provided RNA Loading Dye (2X) and denaturing conditions (N0364)
Protocol
This method utilizes the RNA Loading Dye, (2X) provided and samples should be run on a denaturing polyacrylamide-urea gel prepared with 1X TBE.- Combine on ice:
Low Range ssRNA Ladder (500 μg/ml) 0.5 μl (0.25 μg)
H2O (RNase-free) 4.5 μl
RNA Loading Dye, (2X) 5 μl
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Total Volume 10 μl
- Heat at 65°C for 5 minutes, chill on ice, load
entire sample on gel.
- Run gel according to manufacturer's conditions. All six bands of the marker can be resolved on a 6% gel. 0.5 μg/ml ethidium bromide will effectively stain the bands after electrophoresis.