Perform Second Strand cDNA Synthesis (E7420)
Protocol
- Add the following reagents to the First Strand Synthesis reaction (20 μl):
Nuclease-free Water 48 μl
Second Strand Synthesis Reaction Buffer(10X) 8 μl
Second Strand Synthesis Enzyme Mix 4 μl
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Total volume 80 μl
- Mix thoroughly by gentle pipetting.
- Incubate in a thermal cycler for 1 hour at 16°C, with heated lid set at ≤ 40°C.