Solutions Required for Western Analysis (E8023)
Introduction
10X TBS (per liter):
24.2g Tris base
80g NaCl
Milli-Q™ water
pH 7.6
1X TBS/T (per liter):
100 ml 10X TBS
1 ml Tween-20
800 ml MilliQ water
Blocking Buffer (in 1X TBS/T):
2.5% BSA
2.5% non-fat milk
Primary Antibody Solution:
1:2000 dilution of Anti-GLuc Antibody in Blocking Buffer
HRP-conjugated Secondary Antibody Solution:
1:2000 dilution of Anti-rabbit IgG in Blocking Buffer