FAQ: When using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070), I noticed that wash buffer volumes are the same regardless of the sample input volume. Can I use lower volumes of wash buffers with low sample volume?

For 2 ml and 4 ml sample input, we recommend 1 ml wash volumes. For sample inputs less than 1 ml, reduced volume of the washes can be used if desired, for example, 500-700 µl.