FAQ: What is the maximum binding capacity of the Monarch® Mag Beads M2 provided with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)?

The Monarch® Mag Beads M2 have a binding capacity of up to 5 µg of DNA per prep when using the standard workflow (20 µl of beads) in the DNA cleanup & purification application. This capacity is flexible and can be adjusted by increasing or decreasing the volume of beads used. Protocols are available to support scaling the reaction size up or down, allowing for DNA input either below or above 5 µg, depending on experimental needs.