| Reducing Agents |
Concentration | Activity |
| DTT | 0 mM | 100% |
| 1 mM | 88% | |
| 5 mM | 63% | |
| 10 mM | 46% | |
| 50 mM | 20% | |
| β-ME | 0 mM | 100% |
| 5 mM | 95% | |
| 10 mM | 78% | |
| 20 mM | 75% |
rSAP activity was measured by PNPP assay in 1X rSAP Reaction Buffer supplemented with varying amounts of DTT or β-ME. Activity is shown as percent activity compared to the control, which did not contain added reducing agents.
