Trypsin-ultra, Mass Spectrometry Grade (Recombinant) (NEB #P8119) is recombinantly expressed porcine trypsin manufactured without the use of any animal-derived components. It is mutagenized and further chemically modified via methylation to make the enzyme exceptionally resistant to autolysis. In contrast, Trypsin-ultra, Mass Spectrometry Grade (NEB #P8101), is isolated and purified from bovine pancreas, treated with TPCK to inactivate the copurified chymotrypsin activity, and additionally acetylated to reduce autolysis.
Because it is cloned and expressed in E. coli, Trypsin-ultra, Mass Spectrometry Grade (Recombinant) is naturally free of contaminating chymotrypsin and has enhanced autolysis resistance. These properties improve chromatogram quality and mass spectrometry data, resulting in high-confidence quantitation and identification of peptides. Trypsin-ultra, Mass Spectrometry Grade (Recombinant) is provided in a user-friendly, freeze-dried format, and is stable for 24 months.
