| TEMPERATURE | % ACTIVITY |
|---|---|
| 25°C | < 1% |
| 37°C | < 5% |
| 45°C | 20% |
| 50°C | 50% |
| 60°C | 90% |
| 65°C | 100% |
| 70°C | 95% |
*Incubation time can be increased to obtain enhanced FEN1 cleavage at non-ideal reaction temperatures
| TEMPERATURE | % ACTIVITY |
|---|---|
| 25°C | < 1% |
| 37°C | < 5% |
| 45°C | 20% |
| 50°C | 50% |
| 60°C | 90% |
| 65°C | 100% |
| 70°C | 95% |
*Incubation time can be increased to obtain enhanced FEN1 cleavage at non-ideal reaction temperatures
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