The Bst-XT LAMP/RT-LAMP Master Mix is compatible with a variety of visualization/detection methods including fluorescence, turbidity, and end point colorimetric detection using certain metal-sensing dyes (e.g., calcein with manganese or Eriochrome Black T). This master mix is also compatible with gel-based evaluation and lateral flow methods.
Please note that hydroxynaphthol blue is not recommended for use with this master mix because the resulting color change is not distinct.
If the reaction will be monitored in real time, LAMP Fluorescent Dye (NEB #B1700) can be included at 0.5X final concentration to monitor the reaction in a SYBR/FAM channel of real-time PCR instrument. The dye can be titrated as necessary between 0.1X and 1X.
