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FAQ: Can other proteases be used along with trypsin?

Double protease digestions, performed sequentially or simultaneously, are sometimes used to enhance site-specific cleavage or to achieve maximal coverage of protein sequences. The other protease can be either complementary (e.g., Endoproteinase GluC, NEB #P8100, or α-Lytic Protease, NEB #P8113) or supplementary (e.g., Endoproteinase LysC, NEB #P8109).