HomeFAQsCan the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110) guarantee endotoxin-free plasmid DNA recovery?
FAQ: Can the Monarch® Spin Plasmid Miniprep Kit (NEB #T1110) guarantee endotoxin-free plasmid DNA recovery?
Although Monarch Buffer WZ (Wash Buffer 1) does remove a large majority of endotoxin from the sample, the DNA recovered cannot be guaranteed to be completely free of endotoxin. That said, we have successfully used plasmid DNA recovered using our kits to transfect robust cell lines. While other vendors may claim their minipreps kit are “endotoxin free,” there is usually still some measurable endotoxin.
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