HomeFAQsWhat methods can be used to purify the synthesized protein?
FAQ: What methods can be used to purify the synthesized protein?
Any affinity purification method can be applied, as none of the endogenous proteins in the NEBExpress Cell-free E. coli Protein Synthesis System are tagged. If the synthesized protein contains a His-tag, NEBExpress Ni-NTA Magnetic Beads (NEB #S1423), NEBExpress Ni Spin Columns (NEB #S1427) or NEBExpress Ni Resin (NEB #S1428) can be used. If the synthesized protein contains a maltose-binding protein (MBP) tag, Amylose Resin (NEB #E8021), Amylose Resin High Flow (NEB #E8022) or Amylose Magnetic Beads (NEB #E8035) can be used. If the synthesized protein has a chitin binding domain (CBD) tag, Chitin resin (NEB #S6651) or Chitin Magnetic Beads (NEB #E8036) can be used.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.