FAQ: How much linear DNA template and NEBExpress® GamS Nuclease Inhibitor should I add to my reaction?
During the experimental setup, it is recommended to add the linear DNA template in the last step to allow GamS to bind and inhibit RecBCD before RecBCD has a chance to act on the DNA. In addition, the protein synthesis reaction can be scaled to larger volumes, in which case the amount of linear DNA and GamS should also be scaled proportional to the reaction volume.