HomeFAQsWhat PCR reagents do you recommend for DNA amplification in genome editing (CRIPSR/Cas9,TALEN,ZFN) mismatch detection assays?
FAQ: What PCR reagents do you recommend for DNA amplification in genome editing (CRIPSR/Cas9,TALEN,ZFN) mismatch detection assays?
We recommend Q5® High-Fidelity DNA Polymerase with Q5® Reaction Buffer or Q5 Hot Start High Fidelity 2X Master Mix. The high-fidelity and robustness of Q5 DNA Polymerase makes it a good choice for this assay. The EnGen® Mutation Detection Kit provides reagents for detection of on-target genome editing events. The kit comes with optimized enzyme (including PCR reagents), protocols and includes a control.
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