HomeFAQsWill EDTA interfere with downstream BsaI restriction and ligation?
FAQ: Will EDTA interfere with downstream BsaI restriction and ligation?
No. The NEB Golden Gate Buffer at 1X concentration contains 10 mM MgCl2.
The addition of inserts containing 0.1 mM EDTA will not interfere with the level of MgCl2 required by the enzymes.
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