Glycerol-free and Lyo-compatible Reagents
To support the growing demand for reagents compatible with lyophilization, high-throughput automation, and microfluidic workflows, NEB offers an expanding portfolio of glycerol-free enzyme formulations. Backed by over 50 years of experience in enzyme development and manufacturing, these reagents are designed to deliver high performance while meeting the needs of specialized workflows. Many glycerol-free enzymes are available directly through our catalog for convenient delivery, and additional options, including lyo-compatible mixes for RT-qPCR and LAMP/RT-LAMP, can be accessed via our Customized Solutions team.
This portfolio continues to grow in response to customer needs so check back often for new additions. Don’t see what you’re looking for? Reach out to explore available options, including custom product development tailored to your specific application.
- Contact our Custom Team to access our glycerol-free & lyo-compatible reagents
- Check out our solutions for lyophilization, including ready-to-use lyophilized products for RT-qPCR and LAMP/RT-LAMP
Glycerol-free Products for Molecular Biology Workflows
|
Category |
Product Name |
Recommended Application |
|---|---|---|
|
Reverse Transcriptases |
RT-LAMP |
|
|
Luna® Reverse Transcriptase (Glycerol-free) |
2-step RT-qPCR |
|
|
Luna WarmStart™ Reverse Transcriptase (Glycerol-free) |
1-step RT-qPCR |
|
|
Polymerases |
Hot Start Taq DNA Polymerase (Glycerol-free) |
PCR |
| Taq DNA Polymerase (Glycerol-free) |
PCR |
|
| LAMP and other isothermal amplification | ||
|
Bst-XT DNA Polymerase (Glycerol-free) |
LAMP and other isothermal amplification | |
| LAMP and other isothermal amplification | ||
|
Bst 2.0 DNA Polymerase (Glycerol-free) |
LAMP and other isothermal amplification |
|
|
Bst 3.0 DNA Polymerase (Glycerol-free) |
LAMP and other isothermal amplification |
|
|
Bst DNA Polymerase, Large Fragment (Glycerol-free) |
LAMP and other isothermal amplification |
|
|
Bst DNA Polymerase, Full Length (Glycerol-free) |
Isothermal Amplification |
|
|
Q5® High Fidelity DNA Polymerase (Glycerol-free) |
High-fidelity PCR |
|
|
Q5 Hot Start High-Fidelity DNA Polymerase (Glycerol-free) |
High-fidelity PCR |
|
|
Hemo KlenTaq® (Glycerol-free) |
PCR from blood |
|
|
9°N™ DNA Polymerase (Glycerol-free) |
Incorporation of modified nucleotide |
|
|
Custom Deep Vent® DNA Polymerase (Glycerol-free) |
PCR |
|
|
DNA Modifying Enzymes |
WarmStart Nt.BstNBI (Glycerol-free) |
Nicking Enzyme Amplification Reaction (NEAR) |
|
Lambda Exonuclease (Glycerol-free) |
DNA removal, generation of ssDNA |
|
|
HiFi Taq DNA Ligase (Glycerol-free) |
High-fidelity DNA ligation (e.g., LCR, LDR) |
|
|
T4 DNA Ligase (Glycerol-free) |
DNA ligation |
|
|
T4 Polynucleotide Kinase (Glycerol-free) |
DNA phosphorylation |
|
|
T4 UvsX Recombinase (Glycerol-free) |
Recombinase Polymerase Amplification (RPA) |
|
| T4 UvsY Protein (Glycerol-free) |
Recombinase Polymerase Amplification (RPA) |
|
|
Glycosidases |
Glycan analysis |
|
|
Glycan analysis |
||
|
Other |
Inhibition of RNases |
|
|
T4 Gene 32 Protein (Glycerol-free) |
Recombinase Polymerase Amplification (RPA) and other amplification methods | |
|
ET SSB (Glycerol-free) |
PCR and other amplification methods |
|
|
Thermolabile Proteinase K (Glycerol-free) |
Protein digestion |
|
|
Apyrase (Glycerol-free) |
Dephosphorylation |
|
|
Master Mixes |
WarmStart Multi-Purpose LAMP/RT-LAMP 4X Master Mix (with UDG, Lyo-compatible) |
LAMP/RT-LAMP with carryover prevention |
|
Custom WarmStart LAMP 4X Master Mix (Lyo-compatible) |
LAMP/RT-LAMP |
|
| Luna® One-Step 5X Reaction Mix (Lyo-compatible) |
RT-qPCR |
|
| Luna WarmStart RT 300X Enzyme Mix (with UDG, Lyo-compatible) | RT-qPCR |
Resource Links
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